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Related Experiment Video

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Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

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Published on: November 15, 2019

Developmental switch of intestinal antimicrobial peptide expression.

Sandrine Ménard¹, Valentina Förster, Michael Lotz

¹Swedish Institute for Infectious Disease Control, Karolinska Institute, 171 77 Stockholm, Sweden.

The Journal of Experimental Medicine

|January 9, 2008

Summary

Neonatal intestine uses cathelin-related antimicrobial peptide (CRAMP) for protection against infection. This innate immune peptide is expressed early after birth, then declines as the gut matures.

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Published on: June 24, 2020

Area of Science:

Microbiology
Immunology
Developmental Biology

Background:

Paneth cells produce antimicrobial peptides crucial for gut health, but mature late in development.
Antimicrobial mechanisms protecting the neonatal gut remain largely unknown.

Purpose of the Study:

To investigate the antimicrobial peptide repertoire in the neonatal intestinal epithelium during postnatal development.
To identify novel antimicrobial mechanisms protecting the newborn gut.

Main Methods:

Quantitative reverse transcription-polymerase chain reaction (RT-qPCR)
Immunohistology
Reverse-phase high-performance liquid chromatography (RP-HPLC)
Mass spectrometry

Main Results:

Constitutive expression of cathelin-related antimicrobial peptide (CRAMP) was found in neonatal intestinal epithelium.
Processed, active CRAMP was identified in newborns; synthesis ceased by two weeks postpartum.
CRAMP provided significant protection against Listeria monocytogenes infection in neonatal models.

Conclusions:

Describes a unique developmental switch in innate immune effector expression and localization in the gut.
Epithelial CRAMP expression is vital for early gut colonization, homeostasis, and protection from enteric infections.