Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 8, 2026

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Optimizing BeLPT criteria for beryllium sensitization.

D C Middleton1, J Fink, P J Kowalski

  • 1Agency for Toxic Substances and Disease Registry (ATSDR), Atlanta, Georgia, USA. dcm2@cdc.gov

American Journal of Industrial Medicine
|January 9, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical and acoustic plasmons in the layered material Sr<sub>2</sub>RuO<sub>4</sub>.

Nature communications·2025
Same author

Analysis of endonasal sinus surgery in a private outpatient setting in a tropical environment: A STROBE analysis.

European annals of otorhinolaryngology, head and neck diseases·2024
Same author

Biologically derived epicardial patch induces macrophage mediated pathophysiologic repair in chronically infarcted swine hearts.

Communications biology·2023
Same author

Polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, pesticides, and diabetes in the Anniston Community Health Survey follow-up (ACHS II): single exposure and mixture analysis approaches.

The Science of the total environment·2023
Same author

Novel methods for the assessment of safety pharmacology and toxicology parameters in anesthetized and ventilated dogs receiving inhaled drugs.

Journal of pharmacological and toxicological methods·2019
Same author

Strong Spin Dependence of Correlation Effects in Ni Due to Stoner Excitations.

Physical review letters·2019

The beryllium lymphocyte proliferation test (BeLPT) can confirm beryllium sensitization. Confirming an abnormal BeLPT result with a second test increases accuracy and reduces false positives, especially in low-prevalence populations.

Area of Science:

  • Occupational Medicine
  • Immunotoxicology
  • Diagnostic Test Evaluation

Background:

  • The beryllium lymphocyte proliferation test (BeLPT) is a key diagnostic tool for identifying beryllium sensitization.
  • Expert panels have debated the criteria for establishing beryllium sensitization based on BeLPT results.
  • Three criteria were proposed: one abnormal result, one abnormal and one borderline result, or two abnormal results.

Purpose of the Study:

  • To evaluate and compare the diagnostic performance of three proposed criteria for establishing beryllium sensitization using the BeLPT.
  • To determine the sensitivity, specificity, and positive predictive values (PPVs) for each criterion.

Main Methods:

  • Developed complete algorithms for each of the three proposed BeLPT interpretation criteria.

More Related Videos

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

Related Experiment Videos

Last Updated: Jul 8, 2026

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

  • Utilized pre-existing single-test outcome probabilities to calculate diagnostic metrics.
  • Compared sensitivity, specificity, and PPVs across the different criteria sets.

    • Main Results:

    • All three criteria demonstrated similar overall sensitivity and specificity.
    • Confirmation of an abnormal BeLPT result, via a borderline or second abnormal result, significantly increased PPVs.
    • Confirmation strategies effectively reduced the incidence of false-positive BeLPT results.

    Conclusions:

    • A single, unconfirmed abnormal BeLPT result is generally insufficient to diagnose beryllium sensitization in healthy individuals.
    • In populations with a high prevalence of beryllium sensitization, a single abnormal BeLPT can serve as a strong indicator.
    • Confirmation protocols enhance the reliability of BeLPT for diagnosing beryllium sensitization.