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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

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Characterization of an attenuated total reflection-based sensor for integrated solid-phase extraction and infrared

M Carmen Alcudia-León¹, Rafael Lucena, Soledad Cárdenas

¹Department of Analytical Chemistry, Marie Curie Building (Annex), Campus de Rabanales, University of Cordoba, Cordoba, Spain.

Analytical Chemistry

|January 10, 2008

Summary

A new attenuated total reflection (ATR) sensor combines solid-phase extraction (SPE) for analyte concentration and infrared detection. This integrated system enhances sensitivity for analyzing compounds like caffeine in beverages.

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Area of Science:

Analytical Chemistry
Spectroscopy
Sensor Technology

Background:

Traditional analytical methods often require complex sample preparation.
Integrating sample preparation with detection can streamline analytical workflows.
Attenuated total reflection (ATR) and solid-phase extraction (SPE) are established techniques with potential for synergistic application.

Purpose of the Study:

To develop and validate a novel sensor integrating ATR and SPE for enhanced detection sensitivity.
To demonstrate the sensor's capability for on-line analysis using a sequential injection system.
To assess the performance of the integrated sensor for caffeine determination in soft drinks.

Main Methods:

A novel ATR flow cell was designed to incorporate a SPE sorbent material (LiChrolut EN) directly onto the sensor's sensitive element.
The flow cell was coupled with a sequential injection system for automated solution delivery and spectrum acquisition.
Infrared detection was employed for quantitative analysis after on-line analyte pre-concentration via SPE.
The sensor's performance was validated by determining caffeine in soft drink samples.

Main Results:

The integrated ATR-SPE sensor demonstrated a marked enhancement in sensitivity due to on-line analyte pre-concentration.
Quantitative analysis of caffeine showed good linearity, with established precision (RSD = 4%) and a limit of detection (LOD) of 7 microg/mL.
The system allowed for continuous spectrum acquisition and versatile application without external coating substances.

Conclusions:

The novel ATR-SPE sensor offers a versatile and sensitive platform for on-line chemical analysis.
The integration of SPE within the ATR flow cell significantly improves detection limits.
This approach presents a promising alternative to existing analytical methods for complex sample matrices.