Characterization of the beta-tubulin gene of Pneumocystis carinii
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T D Edlind1, M S Bartlett, J W Smith
1Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
The Journal of Protozoology
|November 1, 1991
Abstract:
To examine the potential of antimicrotubule drugs for treating Pneumocystis carinii infections, and to learn more about this unusual organism on a molecular level, we are studying its tubulin genes. A 0.3 kbp fragment of the P. carinii beta-tubulin gene was amplified by the polymerase chain reaction. Sequence analysis of this DNA revealed that P. carinii beta-tubulin is most closely related to those of the fungal molds. Consistent with these results, P. carinii growth in vitro was sensitive to the antifungal benzimidazoles benomyl and carbendazim.