Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of optical coherence tomography imaging of cataracts with histopathology.

Journal of biomedical optics·2012

Same author

Optical Coherence Tomographic Imaging of Human Tissue at 1.55 μm and 1.81 μm Using Er- and Tm-Doped Fiber Sources.

Journal of biomedical optics·2012

Same author

Changes in the expression of the Alzheimer’s disease-associated presenilin gene in drosophila heart leads to cardiac dysfunction.

Current Alzheimer research·2011

Same author

Blood flow dynamics of one cardiac cycle and relationship to mechanotransduction and trabeculation during heart looping.

American journal of physiology. Heart and circulatory physiology·2011

Same author

Differential coupled mode analysis and the Poincare sphere: erratum.

Applied optics·2010

Same author

Optical spatial tracking using coherent detection in the pupil plane.

Applied optics·2010

Search research articles

Related Experiment Video

Updated: Jul 8, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Optical coherence tomography using a frequency-tunable optical source.

S R Chinn, E A Swanson, J G Fujimoto

|March 1, 1997

Summary

This summary is machine-generated.

Researchers created a fast, high-resolution optical system for imaging. This new optical frequency domain reflectometry system demonstrates advanced depth and resolution capabilities for multidimensional imaging applications.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Related Experiment Videos

Last Updated: Jul 8, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Area of Science:

Photonics and Optical Engineering
Biomedical Imaging
Metrology

Background:

Optical frequency domain reflectometry (OFDR) is a powerful technique for high-resolution measurements.
Traditional OFDR systems can be limited by speed and optical bandwidth.
Multidimensional tomographic imaging requires precise and rapid data acquisition.

Purpose of the Study:

To develop a simple, wide-optical-bandwidth, high-resolution system for rapid OFDR measurements.
To apply the developed system to multidimensional tomographic imaging.
To demonstrate the system's depth and resolution capabilities.

Main Methods:

Utilized a grating-tuned external cavity semiconductor laser with a 25 nm tuning range.
Achieved laser tuning within 100 ms for rapid measurements.
Implemented the system for two-dimensional optical coherence tomography (OCT).

Main Results:

Demonstrated a simple, wide-optical-bandwidth, high-resolution OFDR system.
Successfully applied the system to multidimensional tomographic imaging.
Acquired a two-dimensional OCT image of a thin glass sandwich structure, showcasing depth and resolution.

Conclusions:

The developed OFDR system offers a simple and effective solution for rapid, high-resolution measurements.
The system shows promise for advanced applications in multidimensional tomographic imaging.
Preliminary results confirm the system's capability in demonstrating depth and resolution.