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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

Laser depth measurement based on time-correlated single-photon counting.

J S Massa, A M Wallace, G S Buller

    Optics Letters
    |April 15, 1997
    PubMed
    Summary

    This study introduces a new range data acquisition method using time-correlated single-photon counting. The technique achieves high accuracy, measured at +/-30 micrometers, validated by laboratory experiments and simulations.

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    Published on: December 27, 2018

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    Last Updated: Jul 8, 2026

    Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
    07:00

    Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

    Published on: February 26, 2010

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
    06:08

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

    Published on: December 27, 2018

    Area of Science:

    • Optoelectronics
    • Photonics
    • Metrology

    Background:

    • Accurate range data acquisition is crucial for various scientific and industrial applications.
    • Traditional methods may face limitations in precision or complexity.

    Purpose of the Study:

    • To describe a novel method for acquiring range data.
    • To evaluate the accuracy and performance of this new technique.

    Main Methods:

    • Utilizing time-correlated single-photon counting (TCSPC).
    • Employing a short-pulse laser diode (approx. 10 ps).
    • Using a silicon single-photon avalanche diode detector and standard photon-counting electronics.

    Main Results:

    • The method demonstrated high accuracy in laboratory settings.
    • Measured accuracy was approximately +/-30 micrometers.
    • Experimental results closely matched theoretical simulation predictions.

    Conclusions:

    • The described TCSPC method offers a precise approach for range data acquisition.
    • The technique is validated through experimental measurements and theoretical modeling.
    • This method holds potential for applications requiring high-resolution distance measurements.