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A one-plasmid conditional color-switching transgenic system for multimodal bioimaging

Lei Zheng1, Ching-Ni Njauw, Manuela Martins-Green

  • 1Department of Cell Biology and Neuroscience, University of California-Riverside, Riverside, CA 92521, USA.

Transgenic Research
|January 10, 2008
PubMed
Summary

Researchers created a novel plasmid for generating transgenic mice, enabling controlled gene expression, dual fluorescent markers, bioluminescence, and cell surface tagging for easier cell isolation and imaging.

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...