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Updated: Jul 8, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Studies of polyamines transport through liquid membranes with D2EHPA as a carrier
Katarzyna Dziarkowska1, Katarzyna Koprek, Piotr P Wieczorek
1Institute of Chemistry, Opole University, Oleska, Opole, Poland.
Abstract:
The transport of polyamines through the liquid membranes with di-2-ethylhexyl phosphoric acid (D2EHPA) was investigated. The study was performed in three main steps: liquid-liquid extraction (LLE), bulk liquid membrane (BLM) extraction, and supported liquid membrane (SLM) extraction. Equilibrium distribution experiments allowed determining the extraction constants and stoichiometric coefficients for each polyamine. It turned out that one amino group binds two molecules of carrier (one D2EHPA dimer) and the extractability of polyamine rises with the increase in number of function groups in the molecule. The BLM and SLM experiments showed that despite considerable differences in distribution ratio between various polyamines the extraction efficiencies for all of them are very approximate. The smaller diamines compensate the lowest affinity to membrane phase with faster interface reaction kinetics and higher diffusivity. Finally, the SLM extraction conditions were optimized. The main parameters that influence the transport are the pH of the donor and acceptor phases. The extraction efficiencies obtained for polyamines are high (80-90%) and give hope for an application in bioanalytical chemistry.
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