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Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction
1Department of Chemistry, Banaras Hindu University, Varanasi, India.
Abstract:
Urease has been purified from the seeds of Cajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.
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