[Cloning of the Cf-6 tomato leaf mould resistance locus using SSR markers]

The Cf-6 locus of tomato conferring resistance to the Belarus population of the leaf mould causative agent was mapped to the chromosomal region, located 2.2 and 3.4 cM apart from the microsatellite markers, SSR128 and SSR48, respectively. It was demonstrated that the Cf-6 gene, like the Cf-2/Cf-5 cluster, was located on the short arm of tomato chromosome 6. However, Cf-6 differed from these genes concerning phytopathology and molecular characteristics. Based on the Cf-2 gene sequence, a molecular marker, 2-2C, capable of identification of the Cf-6, Cf-2, and Cf-5 loci, was constructed.