Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-Situ Ligand-Induced Chirality Transfer in Emissive CdSe Nanoplatelets.

The journal of physical chemistry letters·2026
Same author

Long-lived polaritonic coherence and polaron decoupling effects in 2D electronic spectra.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Exploring the Holdase Activity of Supramolecular Chaperones with Amyloid-Forming Peptides and Insulin.

Biomacromolecules·2026
Same author

Depth-Dependent Emission from Silver Dopants in Single CdSe Nanoplatelets.

ACS nano·2026
Same author

Robust photocatalytic hydrogen generation from CdSe nanoplatelets.

Chemical communications (Cambridge, England)·2026
Same author

Analytic rate theory of polariton relaxation that explains long polariton lifetime.

The Journal of chemical physics·2026

Related Experiment Video

Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Flow cytometric analysis to detect pathogens in bacterial cell mixtures using semiconductor quantum dots.

Megan A Hahn1, Peter C Keng, Todd D Krauss

  • 1Department of Chemistry, University of Rochester, Rochester, New York 14627, USA.

Analytical Chemistry
|January 12, 2008
PubMed
Summary

Semiconductor quantum dots (QDs) offer superior bacterial detection over organic dyes in flow cytometry. These quantum dots provide brighter signals and lower detection limits for identifying pathogenic E. coli O157:H7.

More Related Videos

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Related Experiment Videos

Last Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microbiology

Background:

  • Flow cytometry is crucial for analyzing cell mixtures.
  • Organic dyes like fluorescein isothiocyanate are commonly used but have limitations.
  • Accurate detection of pathogenic bacteria is vital for public health.

Purpose of the Study:

  • To compare the efficacy of semiconductor quantum dots (QDs) against organic dyes for analyzing bacterial mixtures.
  • To evaluate the fluorescence intensity, detection limits, and accuracy of QDs in flow cytometry.
  • To assess the potential of QDs for detecting pathogenic E. coli O157:H7.

Main Methods:

  • Utilized CdSe/ZnS core/shell bioconjugates as semiconductor quantum dots (QDs).
  • Employed flow cytometry to analyze mixtures of pathogenic E. coli O157:H7 and harmless E. coli DH5alpha.
  • Compared QD fluorescence intensity and detection limits with fluorescein isothiocyanate.

Main Results:

  • QDs exhibited approximately one order of magnitude brighter fluorescence intensity than fluorescein isothiocyanate.
  • QDs achieved a lower detection limit of 1% E. coli O157:H7 (10^6 cells/mL).
  • QD labeling resulted in greater accuracy for detecting E. coli O157:H7 in mixtures.

Conclusions:

  • Semiconductor quantum dots (QDs) are superior fluorophores for bacterial analysis via flow cytometry.
  • QDs offer enhanced sensitivity and accuracy for pathogen detection compared to traditional organic dyes.
  • The optical properties of QDs present significant advantages for analyzing heterogeneous cell populations.