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Translation Efficiency Test Using Polysome Profiles Under Heat Stress
Published on: October 11, 2024
Transcriptome analysis of acclimatory responses to thermal stress in Antarctic algae
Yong-Sic Hwang1, Gyeongseo Jung, Eonseon Jin
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
Abstract:
A customized cDNA chip analysis provided the relative expression profiling of 1439 ESTs of Chaetoceros neogracile in culture environments maintained between 4 and 10 degrees C. Among the 1439 probes, 21.5% were differentially regulated (2-fold) by the temperature upshift within three days. Up-regulation was more prominent among cytoprotective genes, whereas down-regulation was featured in photosynthetic genes. A third of the differentially expressed genes had an unknown function or no similarity to known genes, highlighting their potential importance as a resource to identify key players in the acclimation response of polar algae under thermal stress. Our transcriptome analysis also revealed novel aspects of temperature-responsive, coordinated changes in the abundance of specific mRNAs, along with the rapid establishment of molecular homeostasis in polar algae. Unexpectedly, a small set of genes encoding fucoxanthin chlorophyll a/c-binding proteins were rapidly up-regulated by thermal stress, implying that they have different roles other than light harvesting.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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