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Updated: Jul 8, 2026
09:08
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
Published on: May 31, 2012
Human protein atlas and the use of microarray technologies
1Department of Proteomics, Albanova University Center, Royal Institute of Technology (KTH), SE-10691 Stockholm, Sweden. sophia.hober@biotech.kth.se
Current Opinion in Biotechnology
|January 12, 2008
Summary
Understanding protein function is crucial for biological research. This study explores genome-wide protein expression profiling using novel affinity ligand-based strategies and microarray technologies, focusing on the Human Protein Atlas initiative.
Area of Science:
- Proteomics and Genomics
- Molecular Biology
- Biotechnology
Background:
- Determining the function of all proteins encoded by an organism's genome is a significant challenge in biological and medical research.
- Genome sequences are increasingly available, enabling systematic approaches to study protein expression.
- Traditional methods focused on mRNA levels, but novel strategies now target protein levels directly.
Purpose of the Study:
- To explore and understand the function of all proteins encoded by an organism's genome.
- To present systematic and high-throughput methods for assessing global protein expression.
- To focus on strategies for profiling protein levels, specifically the Human Protein Atlas initiative and microarray technologies.
Main Methods:
- Development of specific affinity ligands to recognize proteins.
- Utilizing affinity molecules for protein detection across various platforms.
- Application of microarray technologies for protein expression profiling.
Main Results:
- Novel strategies enable direct assessment of protein levels, moving beyond mRNA analysis.
- High-throughput methods allow for systematic global protein expression profiling.
- The Human Protein Atlas initiative leverages these strategies for comprehensive protein information.
Conclusions:
- Genome-wide protein expression profiling is feasible and essential for biological and medical research.
- Affinity ligand-based detection offers a powerful approach for systematic protein analysis.
- Microarray technologies are key tools in advancing protein atlas initiatives and understanding protein function.
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