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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jul 8, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Microarray analysis using disiloxyl 70mer oligonucleotides.

Marcus Gry Björklund1, Christian Natanaelsson, Amelie Eriksson Karlström

  • 1School of Biotechnology, Department of Gene Technology, KTH, Royal Institute of Technology, AlbaNova University Center, SE-10691 Stockholm, Sweden.

Nucleic Acids Research
|January 12, 2008
PubMed
Summary
This summary is machine-generated.

Using full-length DNA microarray probes enhances transcriptome analysis accuracy. A new purification method ensures probe integrity, improving signal detection, especially for low-abundance transcripts, leading to more robust research findings.

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Last Updated: Jul 8, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

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Published on: October 28, 2011

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • DNA microarray technology is a widely used research tool for transcriptomic studies.
  • Advancements have increased reliability, but probe integrity remains a challenge.
  • Truncated probes can lead to inaccurate results due to cross-hybridization and signal loss.

Purpose of the Study:

  • To investigate the impact of full-length probes on transcriptome analysis.
  • To introduce a purification method for ensuring probe integrity.
  • To enhance the reliability and robustness of microarray data.

Main Methods:

  • Analysis of global transcriptome data using DNA microarrays.
  • Introduction and application of a disiloxyl purification step for probes.
  • Comparison of results obtained with full-length versus mixed-length probes.

Main Results:

  • The proportion of full-length probes significantly impacts transcriptome analysis outcomes.
  • Low-abundance transcript measurements are particularly sensitive to truncated probes.
  • The disiloxyl purification step ensures all probes are full-length, increasing signal intensity.

Conclusions:

  • Utilizing full-length probes is crucial for accurate global transcriptome analysis.
  • The disiloxyl purification method effectively enhances probe integrity and signal strength.
  • Increased signal intensity allows for higher hybridization stringency, improving overall data robustness.