Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood proteomics of paediatric bronchiolitis obliterans syndrome after haematopoietic cell transplant.

ERJ open research·2026
Same author

Integrated Molecular, Genomic, and Clinical Characterization of Pediatric and Adolescent Translocation Renal Cell Carcinoma: A Report from the Children's Oncology Group.

Biomedicines·2026
Same author

A lipid-immune network signature defines susceptibility to asparaginase-associated pancreatitis.

JCI insight·2026
Same author

End-to-End Multimodal Multiple Instance Learning for Cancer Histopathology Classification with Dual-Attention Fusion.

Journal of medical systems·2026
Same author

Polysomal Profiling Coupled to Allele-Specific Proteomics Reveals an EIF4H TranSNP Allele Possessing Higher mRNA Translation Potential.

Molecular & cellular proteomics : MCP·2026
Same author

Esomeprazole inhibits proliferation of scleroderma fibroblasts via cell cycle regulation.

Frontiers in pharmacology·2026

Related Experiment Video

Updated: Jul 8, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Functional evolution of the p53 regulatory network through its target response elements.

Anil G Jegga1, Alberto Inga, Daniel Menendez

  • 1Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229-3039, USA.

Proceedings of the National Academy of Sciences of the United States of America
|January 12, 2008
PubMed
Summary

Evolutionary changes in gene regulatory networks are key to biological complexity. This study reveals significant functional differences in p53 response elements across species, impacting stress responses and cancer development.

More Related Videos

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Related Experiment Videos

Last Updated: Jul 8, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Area of Science:

  • Evolutionary biology
  • Genomics
  • Molecular biology

Background:

  • Transcriptional network evolution drives biological complexity.
  • Understanding functional consequences of sequence changes in regulatory elements is crucial.
  • The transcription factor p53 is a master regulator of stress responses.

Purpose of the Study:

  • Investigate the functional evolution of p53 response elements (REs).
  • Analyze sequence and functional conservation of REs across multiple species.
  • Determine the impact of RE evolution on p53-mediated stress responses.

Main Methods:

  • Custom bioinformatics analysis.
  • Multispecies alignment of promoter regions.
  • Functional assessment of p53 REs using established rules and a heat map visualization.

Main Results:

  • Identified orthologous and related p53 REs across 14 species.
  • Observed significant differences in transactivation potential and evolutionary turnover of REs.
  • Found limited functional conservation of p53 REs for DNA repair genes between humans and rodents.

Conclusions:

  • Functional differences in p53 REs are not always predicted by sequence consensus.
  • Widespread evolutionary turnover of REs suggests dynamic regulatory evolution.
  • Lack of functional conservation in DNA repair genes implies species-specific p53 stress response mechanisms and cancer development pathways.