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Rapid active assay for the detection of antibodies to West Nile virus in chickens
Stephanie S Groves1, Michael J Turell, Charles L Bailey
1The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, USA. sgroves1@gmu.edu
Abstract:
To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.
