Multichannel Nomarski microscope with polarization modulation: performance and applications
Related Experiment Video
Updated: Jul 8, 2026
05:54
Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
Published on: September 8, 2023
Optics Letters
|January 12, 2008
Abstract:
Using a two-dimensional CCD array, a photoelastic modulator, and a commercial Nomarski microscope, we built a differential polarization interferometer. Multichannel lock-in detection of the signal allowed us to reach a shot-noise limit corresponding to 5-pm path differences for a few seconds of recording time. This interferometer was used as a surface profilometer as well as a system for imaging through scattering media.
Related Concept Videos
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:29Two-Dimensional Microscopy in Microbiology
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...