Biodegradation of methyl tert-butyl ether as a sole carbon source by aerobic granules cultivated in a sequencing...

Aerobic granules efficient at degrading methyl tert-butyl ether (MTBE) were successfully developed in a well-mixed sequencing batch reactor (SBR). Treatment efficiency of MTBE in the reactor during the stable operations exceeded 99.8%, and effluent MTBE was consistently below 800 microg/L. The specific MTBE degradation rate was observed to increase with increasing MTBE initial concentrations from 25 to 400 mg/L, peaked at 18.2 mg-MTBE/g-VSS h, and declined with further increases in MTBE concentration as substrate inhibition effects became significant. There was a good fit between these biodegradation data and the Haldane equation (R (2) = 0.976). Microbial community DNA profiling was carried out using denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction amplified 16S rDNA. The aerobic granule was found to contain a wide diversity of microorganisms. More than 70% similarity among the samples in the time period examined indicated a highly stable microbial community as the reactor reached the stable operation.