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Updated: Jul 8, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Composite planar electrode for sensing electrochemical oxygen demand
Jahir Orozco1, César Fernández-Sánchez, Ernest Mendoza
1Instituto de Microelectrónica de Barcelona, CNM-IMB (CSIC), Universitat Autònoma de Barcelona, Campus UAB, 08193 Bellaterra, Barcelona, Spain.
Abstract:
This work reports on the development of a graphite-polystyrene composite electrode of planar configuration, containing silver(II) oxide and copper(II) oxide catalysts (AgO-CuO), for the measurement of electrochemical oxygen demand (EOD). Optimisation studies of the composite composition as well as conditions for its processing on planar substrates and generation of an appropriate electrochemical active area resulted in the scalable fabrication of robust composite electrodes. These were evaluated with glucose as target analyte. They showed competitive low limits of detection in a linear concentration range from 5 mgL(-1) to 1400 mgL(-1) of O(2). Besides, they were stable for at least one year. The determination of EOD in wastewater samples coming from production lines of parenteral food and winemaking was successfully carried out.
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