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Related Experiment Video

Updated: Jul 8, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

The microenvironmental determinants for kidney epithelial cyst morphogenesis.

Qiusha Guo¹, Bing Xia, Simon Moshiach

¹Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

European Journal of Cell Biology

|January 15, 2008

Summary

The physical microenvironment significantly impacts epithelial cyst formation. Madin-Darby canine kidney cells form polarized cysts with partial extracellular matrix contact, revealing a novel in vitro model for studying morphogenesis.

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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

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Published on: April 22, 2011

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Last Updated: Jul 8, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Published on: April 22, 2011

Area of Science:

Cell Biology
Developmental Biology
Biophysics

Background:

Epithelial morphogenesis is influenced by both genetic programs and the physical microenvironment.
The specific roles of the physical microenvironment in epithelial morphogenesis remain incompletely understood.
Epithelial cyst formation is a key process in tissue development and a hallmark of certain diseases.

Purpose of the Study:

To investigate the impact of the microenvironment on epithelial cyst formation.
To determine the necessity of extracellular matrix (ECM) presence and cell-ECM interface extent for cystogenesis.
To establish a novel and feasible in vitro model for analyzing epithelial morphogenesis.

Main Methods:

Utilizing Madin-Darby canine kidney (MDCK) cells in a three-dimensional (3D) extracellular matrix (ECM) environment.
Comparing cyst formation under full ECM contact (in-3D) versus partial ECM contact (on-3D) conditions.
Quantitatively analyzing the minimal ECM threshold required for cystogenesis under the on-3D condition.

Main Results:

The presence of ECM in a 3D scaffold is essential for the formation of properly polarized epithelial cysts.
Partial contact between epithelial cells and the ECM (on-3D) is sufficient to induce cystogenesis.
A minimal ECM threshold was defined, above which morphological cues from the ECM trigger cyst formation.
MDCK cells exhibit basal microprotrusions and vesicle production during cystogenesis, correlating with the process.

Conclusions:

The physical microenvironment, specifically the ECM, plays a critical role in regulating epithelial cyst formation.
A partial cell-ECM interface is sufficient for inducing cystogenesis, offering a simplified model for study.
This study quantitatively defines microenvironmental determinants for epithelial cystogenesis and presents a novel in vitro model.