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Updated: Jul 8, 2026

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

An improved cryopreservation method for a mouse embryonic stem cell line.

Corinna M Kashuba Benson¹, James D Benson, John K Critser

¹Comparative Medicine Center, Research Animal Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 1600 East Rollins Street, Columbia, MO 65211, USA.

|January 15, 2008

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Improving cryopreservation of mouse embryonic stem (ES) cells, vital for disease modeling, led to a 2-fold increase in viable cell recovery. This research enhances the storage and transport of these crucial biomedical research tools.

Area of Science:

Cryobiology
Stem Cell Biology
Biomedical Research

Background:

Embryonic stem (ES) cells are essential for creating mouse models of human diseases.
Efficient cryopreservation of ES cells is critical for large-scale mutagenesis projects.
Current cryopreservation methods show high variability in post-thaw recovery rates for mouse ES cells.

Purpose of the Study:

To improve the efficiency and reduce variability in mouse ES cell cryopreservation.
To optimize cryopreservation protocols for C57BL/6 mouse ES cells using fundamental cryobiology principles.

Main Methods:

Characterized membrane permeability and osmotic tolerance of C57BL/6 mouse ES cells.
Used cryobiological data to predict optimal cooling/warming rates and cryoprotectant choice.

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Last Updated: Jul 8, 2026

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Experimentally validated the improved cryopreservation protocol.

Main Results:

Achieved a 2-fold increase in post-thaw recovery of membrane-intact ES cells compared to standard protocols.
Demonstrated a more efficient and reliable method for cryopreserving mouse ES cells.
Propidium iodide exclusion assay confirmed enhanced cell viability.

Conclusions:

The hypothesis-driven cryobiological approach significantly improved mouse ES cell cryopreservation.
This optimized protocol enhances the utility of ES cells for disease modeling and research.
The methodology serves as a model for improving cryopreservation of both mouse and human ES cells.