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Updated: Jul 8, 2026
08:08
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes
Vikas Dighe1, Lisa Clepper, Darlene Pedersen
1Oregon National Primate Research Center, Beaverton, Oregon 97006, USA.
Stem Cells (Dayton, Ohio)
|January 15, 2008
Summary
Monoparental parthenogenetic embryonic stem cells (PESCs) from rhesus monkeys show promise for regenerative medicine. These stem cells can be histocompatible, offering potential for autologous transplantation and disease modeling.
Area of Science:
- Reproductive Biology
- Stem Cell Biology
- Genetics
Background:
- Monoparental parthenotes offer a potential source of histocompatible stem cells.
- Isogenic stem cells are ideal for cell or tissue replacement therapy.
Purpose of the Study:
- To generate and characterize parthenogenetic embryonic stem cell (PESC) lines from rhesus monkeys.
- To assess the suitability of PESC-derived cells for regenerative medicine applications.
Main Methods:
- Generation of five rhesus monkey PESC lines.
- Karyotyping, morphological assessment, and pluripotency marker analysis.
- In vivo and in vitro differentiation assays.
- Analysis of heterozygosity and genomic imprinting.
Main Results:
- Stable, diploid female PESC lines were generated, indistinguishable from controls.
- PESCs expressed key pluripotent markers and differentiated into all three germ layers.
- High heterozygosity was observed, with some lines maintaining egg donor haplotypes.
- Aberrant genomic imprinting was noted in some PESC lines, but this was cell line-dependent.
Conclusions:
- Rhesus monkey PESC lines are practicable to derive and suitable for regenerative medicine.
- PESC derivatives can be used for autologous transplantation or to establish histocompatible cell banks.
- Cell line-dependent variations in imprinting and homozygosity do not preclude PESC utility.
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