Related Experiment Videos
D12S56: a highly polymorphic locus on human chromosome 12q14.
Z Ulinowski1, K Taylor, D Griffin
1Galton Laboratory, University College London, U.K.
Annals of Human Genetics
|October 1, 1991
Summary
Researchers mapped a CA repeat sequence to chromosome 12, specifically at the 12q14 region. This highly polymorphic marker is valuable for genetic mapping and understanding human chromosome structure.
Area of Science:
- Human Genetics
- Molecular Biology
- Genomics
Background:
- Chromosome mapping is crucial for understanding genetic diseases and human evolution.
- Identifying polymorphic markers aids in constructing genetic maps.
- CA repeat sequences are known for their high variability.
Purpose of the Study:
- To assign a specific CA repeat sequence to a human chromosome.
- To determine the precise location of the CA repeat on the identified chromosome.
- To evaluate the utility of this CA repeat as a genetic marker.
Main Methods:
- Subcloning of a CA repeat sequence from a cosmid.
- Somatic cell hybrid panel analysis for chromosome assignment.
- Fluorescent in situ hybridization (FISH) for precise localization.
Main Results:
- The CA repeat sequence was successfully assigned to chromosome 12.
- Further localization refined the site to the 12q14 region.
- The CA repeat was confirmed to be highly polymorphic.
Conclusions:
- The CA repeat sequence is definitively located on chromosome 12 at 12q14.
- This polymorphic marker holds significant potential for genetic mapping applications.
- The findings contribute to the development of more detailed human genetic maps.