Pyrobayes: an improved base caller for SNP discovery in pyrosequences
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Updated: Jul 8, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
Published on: January 8, 2008
Aaron R Quinlan1, Donald A Stewart, Michael P Strömberg
1Department of Biology, Boston College, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA.
Abstract:
Previously reported applications of the 454 Life Sciences pyrosequencing technology have relied on deep sequence coverage for accurate polymorphism discovery because of frequent insertion and deletion sequence errors. Here we report a new base calling program, Pyrobayes, for pyrosequencing reads. Pyrobayes permits accurate single-nucleotide polymorphism (SNP) calling in resequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.
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