Related Experiment Video
Updated: Jul 8, 2026
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
Serum antibody screening by surface plasmon resonance using a natural glycan microarray
Arjen R de Boer1, Cornelis H Hokke, André M Deelder
1Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300, RC Leiden, The Netherlands. a.r.de_boer@lumc.nl
A novel surface plasmon resonance (SPR) glycan microarray enables real-time screening of carbohydrate-binding protein interactions. This SPR array can detect infection-specific antibodies, serving as a potential diagnostic marker for diseases like Schistosoma mansoni.
Area of Science:
- Biochemistry
- Immunology
- Analytical Chemistry
Background:
- Carbohydrate-binding proteins (CBPs) play crucial roles in biological processes.
- Developing efficient methods for screening glycan-protein interactions is essential.
- Natural glycans offer a rich source for understanding these interactions.
Purpose of the Study:
- To develop a surface plasmon resonance (SPR) based natural glycan microarray for screening CBP interactions.
- To demonstrate the array's utility in detecting disease-specific antibodies.
- To establish a method for identifying potential infection markers.
Main Methods:
- Creation of an SPR-based microarray with 144 natural glycan samples.
- Immobilization of glycans via reductive amination and covalent coupling to an epoxide-activated chip.
- Screening of serum samples from Schistosoma mansoni-infected individuals and controls using the SPR array.
Main Results:
- The SPR glycan array allowed real-time, simultaneous screening of CBP interactions without fluorescent labeling.
- The array successfully detected differences in anti-glycan antibody profiles between infected and control sera.
- Sensitive detection of antibody titers and classes (IgG, IgM) was achieved.
Conclusions:
- SPR-based glycan arrays are effective tools for analyzing glycan-CBP interactions.
- These arrays can serve as diagnostic tools for identifying infection status through serum antibody profiling.
- The developed method supports the use of natural or synthetic glycans for biomarker discovery.
