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Parallel expression profiling of barley-stem rust interactions.

Ling Zhang1, Claudia Castell-Miller, Stephanie Dahl

  • 1Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA.

Functional & Integrative Genomics
|January 16, 2008
PubMed
Summary

Barley stem rust resistance gene Rpg1 provides defense against some fungal pathogens. This study identified early gene expression changes in Rpg1-transgenic barley during infection, revealing potential defense signaling components.

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Area of Science:

  • Plant pathology
  • Molecular genetics
  • Agricultural science

Background:

  • The Rpg1 gene in barley confers resistance to numerous strains of the stem rust fungus, Puccinia graminis f. sp. tritici (Pgt).
  • Transgenic barley expressing Rpg1 showed resistance to Pgt pathotype MCC but remained susceptible to Pgt pathotype QCC, indicating partial efficacy.

Purpose of the Study:

  • To identify genes that are upregulated or downregulated during the initial stages of Pgt infection in Rpg1-transgenic barley.
  • To elucidate the molecular mechanisms underlying Rpg1-mediated defense responses against Pgt.

Main Methods:

  • Messenger ribonucleic acid (mRNA) expression analysis was performed using the 22K Barley1 GeneChip.
  • Expression profiling was conducted on two barley lines (susceptible cultivar Golden Promise and Rpg1-transgenic line G02-448F-3R) challenged with two Pgt pathotypes (MCC and QCC) at six time points.

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Main Results:

  • A total of 34 probe sets showed differential expression between susceptible and resistant barley lines infected with Pgt-MCC.
  • Fourteen probe sets displayed altered expression when comparing an avirulent (Pgt-MCC) to a virulent (Pgt-QCC) pathotype on the Rpg1-transgenic line.
  • These differentially expressed genes were activated early in the infection process, preceding visible defense responses.

Conclusions:

  • The study identified candidate genes involved in early signaling pathways during Rpg1-mediated resistance.
  • These candidate genes offer potential targets for further functional analysis to understand barley's defense mechanisms against stem rust.