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Updated: Jul 8, 2026

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Published on: September 26, 2013

Human adipose stem cells: a potential cell source for cardiovascular tissue engineering.

Sepideh Heydarkhan-Hagvall¹, Katja Schenke-Layland, Jin Q Yang

¹Regenerative Bioengineering and Repair Laboratory, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, Calif., USA.

Cells, Tissues, Organs

|January 17, 2008

Summary

This summary is machine-generated.

Human adipose stem cells (hASCs) show potential for cardiovascular tissue engineering. Their differentiation into smooth muscle cells and endothelial cells depends on passage number and culture conditions.

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Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Published on: March 5, 2019

Area of Science:

Cardiovascular Tissue Engineering
Stem Cell Biology
Regenerative Medicine

Background:

Identifying optimal cell sources is critical for cardiovascular tissue engineering applications.
Human adipose stem cells (hASCs) are being investigated as a potential cell source.
Understanding differentiation parameters is key to their clinical relevance.

Purpose of the Study:

To identify critical exogenous and endogenous parameters for differentiating hASCs into cardiovascular cells.
To evaluate hASCs for their potential in cardiovascular tissue engineering.

Main Methods:

hASCs were isolated from human lipoaspirate.
Cells were subjected to two distinct differentiation protocols.
Characterization involved fluorescence-activated cell sorter (FACS) analysis, immunofluorescence, and scanning electron microscopy.

Main Results:

hASCs expressed key stem cell markers (CXCR4, CD34, c-kit, ABCG2).
Differentiation protocols induced expression of smooth muscle cell (SMC)-specific markers (SM alpha-actin, etc.) and endothelial cell (EC)-specific markers (CD31, CD144, von Willebrand factor).
hASC differentiation capacity was dependent on cell passage number and culture medium (DMEM-20%-FBS for SMCs, EGM-2 for ECs).
hASCs integrated with collagen-elastin scaffolds, forming a cell-matrix network.

Conclusions:

hASCs represent a promising cell source for cardiovascular tissue engineering.
The differentiation potential of hASCs into SMCs and ECs is influenced by passage number and culture conditions.
Further optimization of culture parameters is necessary for maximizing hASC differentiation for therapeutic applications.