A specific, sensitive and high-capacity immunoassay for PAF
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B A Baldo1, M A Smal, A C McCaskill
1Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St. Leonards, N.S.W., Australia.
Lipids
|December 1, 1991
Abstract:
A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.