Efficient subpixel image registration algorithms
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Updated: Jul 8, 2026

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
Published on: October 27, 2023
Manuel Guizar-Sicairos1, Samuel T Thurman, James R Fienup
1The Institute of Optics, University of Rochester, Rochester, New York, 14627, USA.
Abstract:
Three new algorithms for 2D translation image registration to within a small fraction of a pixel that use nonlinear optimization and matrix-multiply discrete Fourier transforms are compared. These algorithms can achieve registration with an accuracy equivalent to that of the conventional fast Fourier transform upsampling approach in a small fraction of the computation time and with greatly reduced memory requirements. Their accuracy and computation time are compared for the purpose of evaluating a translation-invariant error metric.

