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Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
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Related Experiment Video

Updated: Jul 8, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Automated oligosaccharide synthesis.

Peter H Seeberger¹

¹Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zürich, HCI F 315, Wolfgang-Pauli-Str. 10, CH-8093 Zürich, Switzerland. seeberger@org.chem.ethz.ch

Chemical Society Reviews

|January 17, 2008

Summary

Automated solid-phase synthesis now enables non-specialists to create complex carbohydrates, accelerating research and development for applications like the malaria vaccine.

Area of Science:

Carbohydrate chemistry
Polymer science
Biotechnology

Background:

Automated synthesis is established for peptides and oligonucleotides, but challenging for complex carbohydrates.
Growing recognition of carbohydrate biopolymers' biological significance drives demand for efficient synthesis methods.

Purpose of the Study:

To review the current state of automated solid-phase oligosaccharide synthesis.
To identify key areas for future innovation in carbohydrate assembly.

Main Methods:

Review of automated solid-phase synthesis techniques for oligosaccharides.
Discussion of advancements and limitations in current methodologies.

Main Results:

Automated solid-phase synthesis is becoming increasingly viable for complex carbohydrate structures.

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High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

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Last Updated: Jul 8, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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Published on: September 6, 2019

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

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Specific innovations are needed to overcome existing challenges in efficiency and scope.

Conclusions:

Automated synthesis offers a promising path for producing complex carbohydrates, including for vaccine development.
Further advancements are crucial to fully realize the potential of automated oligosaccharide synthesis.