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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
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ADSA-TRIS: a new method to study interfacial phenomena at polymer-aqueous solution interfaces.

Kerstin Nötzold1, Stefanie Jäger, Stefan Michel

  • 1Faculty of Materials Science and Materials Technology, TU BA Freiberg, G.-Zeuner-Str. 5, 09599, Freiberg, Germany.

Analytical and Bioanalytical Chemistry
|January 17, 2008
PubMed
Summary

This study introduces a novel method combining Axisymmetric Drop Shape Analysis-Profile (ADSA-P) and Total Reflectometric Interference Spectroscopy (TRIS) to analyze interfacial changes. The technique monitors protein adsorption and conformational shifts on polymer surfaces in real-time.

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Area of Science:

  • Surface Science and Interfacial Phenomena
  • Materials Science
  • Biophysics

Background:

  • Studying interfacial phenomena requires precise measurement of dynamic changes at solid-liquid and liquid-vapor interfaces.
  • Adsorption and desorption of surface-active substances, like proteins, significantly alter interfacial properties.
  • Existing methods may lack the simultaneous capability to monitor both interfacial tension and film thickness changes.

Purpose of the Study:

  • To develop and validate a combined experimental setup integrating ADSA-P and TRIS for interfacial analysis.
  • To investigate interfacial phenomena on polymer surfaces caused by the adsorption/desorption of surface-active substances.
  • To monitor protein adsorption kinetics and conformational changes on hydrophobic polymer surfaces.

Main Methods:

  • Combined Axisymmetric Drop Shape Analysis-Profile (ADSA-P) and Total Reflectometric Interference Spectroscopy (TRIS) in a single experimental setup.
  • Utilized sessile liquid droplets on polymer films (polystyrene, poly(4-hydroxystyrene)) on chromium-coated glass substrates.
  • Simultaneously analyzed droplet shape (via ADSA-P) and optical film thickness (via TRIS) to determine interfacial parameters over time.

Main Results:

  • The combined ADSA-P and TRIS method successfully monitored time-dependent changes in solid-vapor interfacial tension, contact angle, contact radius, drop volume, and solid-liquid interfacial tension.
  • Observed time-dependent changes in optical film thickness correlated with adsorption of human serum albumin (HSA) and lysozyme (LSZ).
  • Changes in optical film thickness were accompanied by alterations in solid-liquid interfacial tension, indicating protein adsorption and potential conformational changes.

Conclusions:

  • The integrated ADSA-P and TRIS technique provides a powerful tool for real-time interfacial studies.
  • Demonstrated the method's efficacy in studying protein adsorption kinetics (HSA, LSZ) on hydrophobic polystyrene surfaces.
  • The study provides insights into protein conformational changes occurring within adsorbed layers on polymer surfaces.