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Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Published on: January 28, 2013
Improvement in retinol analysis by fluorescence and solid phase extraction (SPE) in micellar medium
M Torre1, M Sánchez-Hernández, S Vera
1Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Carretera Madrid-Barcelona km 33,6, Alcalá de Henares, Madrid, Spain.
Journal of Fluorescence
|January 17, 2008
Summary
This study developed a sensitive fluorescence method for determining retinol (vitamin A) in aqueous solutions. Solid phase extraction combined with fluorescence offers a fast and accurate technique for retinol analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Retinol, the most active form of vitamin A, requires precise analytical methods for accurate determination.
- Traditional methods for retinol analysis can be complex and less sensitive.
Purpose of the Study:
- To establish a sensitive and efficient method for retinol determination.
- To explore the use of aqueous micellar media and solid phase extraction (SPE) for retinol analysis.
Main Methods:
- Retinol determination using fluorescence spectroscopy in an aqueous micellar medium.
- Application of solid phase extraction (SPE) to modify the sample matrix.
- Analysis of organic samples using a CTAB/n-butanol/water mixture.
Main Results:
- The fluorescence method achieved high sensitivity with a detection limit of 0.03 mg L(-1).
- Retinol remained stable in solution for at least 5 days.
- SPE achieved nearly 100% recovery for retinol-spiked samples, facilitating matrix modification.
Conclusions:
- The combined SPE and fluorescence technique provides a sensitive, fast, and effective method for simultaneous retinol identification and determination.
- This approach enhances analytical efficiency and sensitivity compared to other media.
