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Related Experiment Video

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Nanoscale affinity chip interface for coupling inhibition SPR immunosensor screening with Nano-LC TOF MS.

G R Marchesini1, J Buijs, W Haasnoot

  • 1RIKILT-Institute of Food Safety, Wageningen UR, Wageningen, The Netherlands. gerardo.marchesini@wur.nl

Analytical Chemistry
|January 17, 2008
PubMed
Summary

A novel nanoscale biosensor system integrates surface plasmon resonance (SPR) with nano-liquid chromatography mass spectrometry (nano-LC ESI TOF MS). This allows rapid screening and identification of low molecular weight compounds like enrofloxacin in food samples.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Food Safety

Background:

  • Screening low molecular weight compounds requires sensitive and specific analytical methods.
  • Existing methods for food analysis can be time-consuming and complex.
  • On-line coupling of biosensors with mass spectrometry offers potential for improved efficiency.

Purpose of the Study:

  • To develop and validate an on-line nanoscale coupled system for screening and identification of low molecular weight molecules.
  • To integrate a surface plasmon resonance (SPR)-based inhibition biosensor immunoassay (iBIA) with nano-liquid chromatography electrospray ionization time-of-flight mass spectrometry (nano-LC ESI TOF MS).
  • To demonstrate the system's capability for analyzing incurred food samples.

Main Methods:

  • Development of a reusable recovery chip (RC) with a hydrogel-modified biosorbent for analyte capture.
  • Utilizing an SPR-based biosensor for performance characterization and screening.
  • Employing nano-LC ESI TOF MS for identification of captured analytes after elution.
  • A four-stage procedure: sample preparation, screening, analyte capture on RC, and MS analysis.

Main Results:

  • The coupled system effectively screened enrofloxacin in incurred chicken muscle samples.
  • Identity confirmation of enrofloxacin and identification of its metabolite, ciprofloxacin, were achieved.
  • The system demonstrated subnanogram level analyte capture capacity.
  • A robust triple-chip system combining screening chip (SC), recovery chip (RC), and nano-LC chip TOF MS was demonstrated.

Conclusions:

  • The on-line nanoscale coupled biosensor and mass spectrometry system provides a robust alternative for rapid screening and identification.
  • This technology holds potential for detecting both known and unknown compounds in complex matrices.
  • The reusable recovery chip facilitates efficient analyte capture and analysis, enhancing workflow efficiency.