Related Experiment Video
Updated: Jul 8, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
A Study of the interaction of HEK-293 cells with streamline chelating adsorbent in expanded bed operation
Frédéric Poulin1, Renaud Jacquemart, Gregory De Crescenzo
1Biotechnology and pharmaceutical processes research unit (Bio-P2), Department of Chemical Engineering, Ecole Polytechnique, Montréal, Québec, Canada.
Abstract:
Expanded bed adsorption (EBA) is an efficient protein purification process reducing time and steps of downstream processing (DSP) since nonclarified culture media can be processed directly without prior treatments such as filtration or centrifugation. However, cells and debris can interact with the adsorbent and affect bed stability as well as purification performance. To optimize EBA operating conditions these biomass/adsorbent interactions have to be understood and characterized. The adsorption of Human Embryonic Kidney cells (HEK 293) on unprimed and nickel-primed metal affinity adsorbent was studied in a closed loop EBA setup. With the unprimed adsorbent, the overall level of interaction observed was nonsignificant. With the nickel-primed adsorbent and an initial cell concentration ranging from 0.08 x 10(6) to 0.2 x 10(6) cells/mL, biomass/adsorbent interaction was found to be moderate and the adsorption apparent first-order kinetic rate constant was determined to be k = 0.009 to 0.011 min(-1).
More Related Videos
Related Concept Videos
Ion Exchange
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...

