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Published on: February 16, 2022
Xerogel optical sensor films for quantitative detection of nitroxyl
Kevin P Dobmeier1, Daniel A Riccio, Mark H Schoenfisch
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Analytical Chemistry
|January 17, 2008
Summary
New xerogel films detect nitroxyl (HNO) using a manganese porphyrin. Enhanced permeability allows for sensitive and selective HNO quantification, aiding in the study of HNO donors.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biomedical Engineering
Background:
- Nitroxyl (HNO) is a signaling molecule with therapeutic potential.
- Accurate detection of HNO is crucial for understanding its biological roles and developing HNO-based therapies.
- Existing methods for HNO detection face challenges in sensitivity, selectivity, and real-world application.
Purpose of the Study:
- To develop novel optical sensors for selective nitroxyl (HNO) detection.
- To enhance the performance of HNO sensors through material modification.
- To quantify HNO generation from different donors and assess pH dependency.
Main Methods:
- Sol-gel chemistry was employed to synthesize xerogel sensing films.
- Manganese(III) meso-tetrakis(4-sulfonatophenyl) porphyrinate (MnIIITPPS) was used as the indicator for HNO detection.
- Dendrimers were incorporated into the xerogel matrix to improve nitroxyl permeability.
- Optical detection was based on monitoring the reductive nitrosylation of MnIIITPPS.
Main Results:
- Selective detection of HNO in solution was achieved using the fabricated xerogel films.
- Incorporation of dendrimers significantly enhanced nitroxyl permeability in the sensor films.
- The sensors demonstrated good selectivity, sensitivity, and a defined working dynamic range for HNO.
- The microtiter plates successfully quantified pH-dependent HNO generation from IPA/NO and compared it to Angeli's salt.
Conclusions:
- The developed xerogel-based optical sensors offer a promising platform for selective and sensitive HNO detection.
- The enhanced permeability via dendrimer incorporation improves sensor performance for practical applications.
- These sensors facilitate the study of HNO generation mechanisms and the evaluation of HNO-donating compounds.
