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Published on: April 4, 2019
Inhibition of gene expression directed by small interfering RNAs in infectious bronchitis virus
1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, PR China.
Abstract:
In this study, fourteen small interfering RNAs (siRNAs) were synthesized for targeting of coding gene sequences, specific 5'-leader sequence of mRNA and 3'-end sequence of the Infectious bronchitis virus (IBV) genome. The expression of viral genes in Vero-E6 cells was detected by real-time PCR. Obtained results indicated that the majority of siRNAs could effectively inhibit the expression of viral genes. The inhibition effect of siRNAs significantly differed among various genes and sites on the virus genome.
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