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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

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UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
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Published on: October 25, 2021

Nondestructive varnish identification by ultraviolet fluorescence spectroscopy.

Mathieu Thoury1, Mady Elias, Jean Marc Frigerio

  • 1Institut des Nano-Sciences de Paris, UMR 7588 CNRS, Université Pierre et Marie Curie, Campus Boucicaut, 140 rue de Lourmel 75015 Paris, France.

Applied Spectroscopy
|January 17, 2008
PubMed
Summary

Art restorers can now identify varnish types non-destructively using ultraviolet (UV) fluorescence spectroscopy. This real-time, in situ method aids in selecting appropriate solvents for conservation, improving art preservation techniques.

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Area of Science:

  • Art conservation science
  • Analytical chemistry
  • Spectroscopy

Background:

  • Accurate varnish identification is crucial for art restorers to select appropriate solvents for conservation treatments.
  • Traditional chemical analysis requires sampling, which can be invasive and time-consuming.
  • Developing non-destructive, in situ methods is essential for preserving delicate artworks.

Purpose of the Study:

  • To introduce and validate ultraviolet (UV) fluorescence spectroscopy as a novel, non-destructive technique for identifying varnish composition.
  • To enable real-time, in situ analysis of varnishes on artworks.
  • To provide art conservators with a tool for informed solvent selection.

Main Methods:

  • Utilized ultraviolet (UV) fluorescence spectroscopy for varnish analysis.
  • Compared emission spectra of unknown varnishes with reference samples (fresh, aged, natural resins).
  • Employed monochromatic excitation wavelengths to differentiate varnish types (spirit, oil, mixed).

Main Results:

  • UV fluorescence spectroscopy successfully differentiated between various resin and varnish types.
  • The method proved effective for both artificial and naturally aged samples.
  • Successful application demonstrated on real works of art and samples on fluorescent backgrounds.

Conclusions:

  • Ultraviolet (UV) fluorescence spectroscopy is a viable, non-destructive technique for in situ varnish identification in art conservation.
  • This method allows for rapid, real-time analysis, aiding conservators in choosing correct solvents.
  • The technique enhances the ability to preserve cultural heritage by informing conservation decisions.