Related Experiment Video
Updated: Jul 8, 2026
04:44
Near-Infrared Spectroscopy During Reactive Hyperemia for the Assessment of Lower Limb Vascular Function
Published on: March 22, 2024
Method optimization on the use of postocclusive hyperemia model to assess microvascular function
Get Bee Yvonne-Tee1, Aida Hanum Ghulam Rasool, Ahmad Sukari Halim
1Department of Biomedicine, School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Malaysia. yvonne@kb.usm.my
Clinical Hemorheology and Microcirculation
|January 17, 2008
Summary
Optimizing microvascular function assessment requires tailored occlusion durations for postocclusive reactive hyperemia (PORH) max, varying by age, gender, and menstrual phase. However, time-to-peak (Tp) measurements are unaffected by these factors.
Area of Science:
- Physiology
- Vascular Biology
- Non-invasive Diagnostics
Background:
- Microvascular function assessment in vivo is advancing but lacks standardization.
- Laser Doppler fluximetry (LDF) is a key tool for non-invasive evaluation.
- Postocclusive reactive hyperemia (PORH) is a critical indicator of microvascular response.
Purpose of the Study:
- To determine the optimal occlusion duration for eliciting reliable PORH responses in forearm skin.
- To investigate the influence of age, gender, and menstrual phase on PORH parameters.
- To standardize PORH assessment using LDF for improved microvascular function analysis.
Main Methods:
- 120 healthy subjects (21-50 years, both genders) participated.
- Occlusion durations of 1, 2, or 3 minutes were applied.
- Skin perfusion was measured using LDF, analyzing PORH max and time-to-peak (Tp).
Main Results:
- PORH max is significantly influenced by age, gender, and menstrual phase.
- A 2-minute occlusion was effective for females during the high estrogenic phase.
- Tp measurements showed significant changes with 3-minute occlusion across all groups, irrespective of other factors.
Conclusions:
- Occlusion duration for PORH max assessment must account for individual factors like age, gender, and menstrual phase.
- Tp measurement offers a standardized approach for microvascular assessment, independent of these variables.
- Standardized PORH+LDF protocols are crucial for accurate microvascular function evaluation.
