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Updated: Oct 7, 2026

Techniques for Imaging Ca2+ Signaling in Human Sperm
Published on: June 17, 2010
Fluorometric labeling of tetrahydroprogesterones
1Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Abstract:
7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards and steroids from biologic mixtures were separated and showed similar characteristics. The methods used are described.
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