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Updated: Jul 8, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Pharmaceutical applications of isoelectric focusing on microchip with imaged UV detection
Markéta Vlcková1, Franka Kalman, Maria A Schwarz
1Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Abstract:
For the first time, the application of a commercial Shimadzu microchip electrophoresis system MCE-2010 equipped with an imaging UV detector for isoelectric focusing (IEF) of therapeutic proteins is reported. By proper adjustment of the pH gradient, samples with pI values ranging from 2.85 to 10.3 can be focused to the imaged part of the separation channel. Three therapeutic proteins (hirudin, erythropoietin, and bevacizumab) have been successfully focused on the microchip, and the results have been compared to conventional capillary IEF in terms of peak profile, pI values, and reproducibility.
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