Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Column Efficiency: Rate Theory

Column Efficiency: Rate Theory

The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...

Chromatography: Introduction

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

Chromatographic Methods: Terminology

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...

Diffusion on Chromatography Columns

Diffusion on Chromatography Columns

In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...

Chromatographic Methods: Classification

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...

Principles Of Column Chromatography

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lévy Formulation of the Stochastic Theory of Chromatography and Extension to Phase-Type Markov Renewal Process.

Analytical chemistry·2026

Same author

Phase-Specific Parameter Estimation in Chiral HPLC Using 1-Site, 2-Site Stochastic Models, and Unified Equation Approach.

Analytical chemistry·2026

Same author

Application of design of experiments to optimize the separation of lipid-related compounds in supercritical fluid chromatography-mass spectrometry.

Journal of chromatography. A·2025

Same author

Automated R workflow for Batman peak fitting using unified equation and stochastic modeling.

Journal of chromatography. A·2025

Same author

Mathematical modeling of Taylor-Aris dispersion-assisted mass spectrometry for the study of operating conditions.

Talanta·2025

Same author

Retention mechanism on phosphodiester stationary phases in HILIC and purely aqueous mobile phase, Part I: The problem of hold-up volume determination.

Journal of chromatography. A·2025

Search research articles

Related Experiment Video

Updated: Jul 8, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Molecular dynamic theories in chromatography.

Attila Felinger¹

¹Department of Analytical and Environmental Chemistry, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary. felinger@ttk.pte.hu

Journal of Chromatography. A

|January 18, 2008

Summary

This summary is machine-generated.

This study details a molecular dynamic model for chromatography, explaining molecule migration and adsorption. This model effectively characterizes heterogeneous stationary phases in various chromatographic separations.

More Related Videos

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

Related Experiment Videos

Last Updated: Jul 8, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

Area of Science:

Analytical Chemistry
Physical Chemistry

Background:

Chromatography relies on molecular interactions within mobile and stationary phases.
Microscopic models are essential for understanding complex separation dynamics.

Purpose of the Study:

To present a molecular dynamic model for chromatography.
To describe the fundamental processes governing chromatographic separations at a molecular level.

Main Methods:

Modeling molecular migration in the mobile phase using a one-dimensional random walk.
Simulating adsorption-desorption on the stationary phase via a Poisson process.
Assuming exponential sojourn times for molecules in both phases.

Main Results:

The molecular dynamic model captures the essential behaviors of chromatographic systems.
The model is applicable to heterogeneous stationary phases, enhancing characterization.
Demonstrated utility across reversed-phase, chiral, size-exclusion, and ion-exchange chromatography.

Conclusions:

The molecular dynamic model provides a robust framework for understanding chromatographic separations.
This microscopic approach is valuable for analyzing heterogeneous stationary phases.
The model's versatility supports diverse chromatographic applications.