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Non-invasive Imaging of Leukocyte Homing and Migration in vivo
Published on: December 6, 2010
Human leukocyte migration inhibition factor (LIF) increases polymorphonuclear cell endocytosis
M A Cortés-Castillo1, L Favila-Castillo
1Departamento de Inmunologia, Escuela Nacional de Ciencias Biológicas, México, D.F.
Archivos De Investigacion Medica
|January 1, 1991
Summary
Leukocyte migration inhibition factor (LIF) enhances the ability of polymorphonuclear (PMN) cells to engulf yeast particles. This finding suggests LIF plays a role in modulating immune cell endocytic activity.
Area of Science:
- Immunology
- Cell Biology
Background:
- Leukocyte migration inhibition factor (LIF) is a cytokine produced by activated lymphocytes.
- The precise role of LIF in modulating immune cell function, particularly phagocytosis, requires further elucidation.
Purpose of the Study:
- To investigate the effect of purified leukocyte migration inhibition factor (LIF) on the endocytic activity of polymorphonuclear (PMN) cells.
- To determine if LIF enhances yeast uptake by PMN cells.
Main Methods:
- Preparation of supernatants from Concanavalin-A activated human lymphocytes containing LIF.
- Fractionation of supernatants using Sephadex G-100 chromatography.
- Assay of LIF activity using a yeast endocytosis model with human PMN cells.
- Inhibition studies using N-acetyl-D-glucosamine, a specific LIF blocker.
Main Results:
- A specific fraction containing high LIF activity was isolated from activated lymphocyte supernatants.
- This LIF-containing fraction significantly increased the number of endocytic PMN cells by approximately 80%.
- The observed enhancement of endocytosis was specific to LIF and blocked by N-acetyl-D-glucosamine.
Conclusions:
- Leukocyte migration inhibition factor (LIF) demonstrably enhances the endocytic capacity of polymorphonuclear (PMN) cells.
- LIF may play a crucial role in regulating phagocytic immune responses.
