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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
Published on: May 28, 2015
Expression of GABArho subunits during rat cerebellum development
Carmen Mejía1, Guadalupe García-Alcocer, Laura C Berumen
1Instituto de Neurobiología, Departamento de Neurobiología Molecular y Celular, Universidad Nacional Autónoma de México-Campus Juriquilla, (Boulevar) Universitario 3001, Querétaro, 76230, Mexico.
Abstract:
In the present study, we provide evidence for the expression of all three GABA(C) receptor rho subunits through development of the rat cerebellum. Injection of cerebellum mRNA into frog oocytes gave rise to the expression of both GABA(A) and GABA(C) receptors. qRT-PCR of RNA isolated from postnatal developing cerebella showed that the expression of each rho subunit is relatively low, with a relative comparative expression of rho3>rho1>rho2. In situ hybridization and immunohistochemistry revealed a limited distribution of GABA(C) receptors in the Purkinje and Golgi neurons whereas electron microscopy detected the rho1 and rho2 subunits in the soma and dendritic tree of the Purkinje cells. The expression of GABA(C) receptors in the cerebellum adds a new dimension to the regulation of GABAergic neurotransmission and suggests further experiments to determine their functional consequences.
