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Updated: Jul 8, 2026
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
Published on: May 25, 2018
Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in
Nirmala Bardiya1, William G Alexander, Tony D Perdue
1Division of Biological Sciences, University of Missouri, Columbia 65211, USA.
Abstract:
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.
