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An Orthotopic Murine Model of Human Prostate Cancer Metastasis
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Published on: September 18, 2013

Resveratrol down-regulates the androgen receptor at the post-translational level in prostate cancer cells

Naoki Harada1, Yohei Murata, Ryoichi Yamaji

  • 1Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.

Journal of Nutritional Science and Vitaminology
|January 19, 2008
PubMed

Insights

Resveratrol inhibits prostate cancer progression by reducing androgen receptor (AR) levels. This study shows resveratrol decreases AR protein stability, impacting its function through post-translational mechanisms.

Area of Science:

  • Molecular Biology
  • Oncology
  • Nutraceuticals

Background:

  • Androgen receptor (AR) is a key factor in prostate cancer development and progression.
  • Resveratrol is known to inhibit AR function and expression transcriptionally.

Purpose of the Study:

  • To investigate the effects of resveratrol on AR function and post-translational AR levels.
  • To elucidate the mechanism by which resveratrol impacts AR activity in prostate cancer cells.

Main Methods:

  • Assessed resveratrol's effect on wild-type and mutant AR transcriptional activity in PC-3 cells.
  • Determined AR protein half-life using cycloheximide chase in LNCaP cells with and without resveratrol.

Main Results:

  • Resveratrol repressed AR transcriptional activity independently of the ligand-binding domain.
  • Resveratrol significantly reduced AR protein half-life from approximately 13 h to 4 h.
  • This indicates resveratrol down-regulates AR protein via a post-translational mechanism.

Conclusions:

  • Resveratrol inhibits AR function partly by decreasing post-translational AR protein levels.
  • The findings suggest a novel therapeutic strategy for prostate cancer utilizing resveratrol.

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