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Oligopeptide Competition Assay for Phosphorylation Site Determination
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Peptide microarray for high-throughput determination of phosphatase specificity and biology

Hongyan Sun1, Candy H S Lu, Mahesh Uttamchandani

  • 1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Angewandte Chemie (International Ed. in English)
|January 19, 2008
PubMed
Abstract

No abstract available in PubMed .

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Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

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