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Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
Bacterial communities of Bartonella-positive fleas: diversity and community assembly patterns
Ryan T Jones1, Katherine F McCormick, Andrew P Martin
1Department of Ecology and Evolutionary Biology, CB334, University of Colorado-Boulder, Boulder, Colorado 80309, USA. Ryan.Jones@Colorado.edu
Abstract:
We investigated the bacterial communities of nine Bartonella-positive fleas (n = 6 Oropsylla hirsuta fleas and n = 3 Oropsylla montana fleas), using universal primers, clone libraries, and DNA sequencing. DNA sequences were used to classify bacteria detected in a phylogenetic context, to explore community assembly patterns within individual fleas, and to survey diversity patterns in dominant lineages.
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