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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Sensitive spectrophotometric methods for the assessment of nitrite in water sample
P Nagaraj1, J S Prakash, Anantharaman Shivakumar
1Department of Studies in Chemistry, University of Mysore, Manasagangothri, Mysore, 570006, India. nagarajap58@yahoo.co.in
Abstract:
Two spectrophotometric methods have been developed for the determination of nitrite using dapsone (DAP) with alpha-naphthol and 4-amino-5-hydroxynapthalene-2,7-disulphonic acid monosodium salt (AHNDMS) as chromogenic reagents with maximum absorbance wavelength at 540 and 520 nm respectively. For the method that utilizes dapsone with alpha-naphthol (DAP-alpha-naphthol), the beer's law range is obeyed between 0.05-0.8 microg ml(-1) with molar absorptivity of 5.749 x 104 l mol(-1) cm(-1). The second method that uses dapsone with AHNDMS (DAP-AHNDMS), the beer's law is valid over the range 0.2-1.4 mug ml(-1) and molar absorptivity 2.44 x 104 l mol(-1) cm(-1). The common interfering ions in the analytical procedures have been studied. This proposed methods are reliable, reproducible and have been successfully applied to determine nitrite in various water sources of environmental interest.
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