Synthesis of oligonucleotide derivatives using ChemMatrix supports
Stefania Mazzini1, Fayna García-Martin, Margarita Alvira
1Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Spain.
Abstract:
The synthesis of oligonucleotides on poly(ethylene glycol)-based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthesis of oligonucleotide-peptide conjugates and for the ligation of oligonucleotides using Cu(+)-catalyzed cycloadition reactions is reported. Moreover, these supports can be used for the preparation of oligonucleotides in anhydrous solvents, followed by hybridization of the complementary sequences in aqueous buffers.
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