Related Experiment Video

Updated: Jul 8, 2026

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

[Hyperplasia suppressor gene expression in vascular smooth muscle cells derived from normotensive and hypertensive

Ya-ping Liu1, Shao-jun Wen, Ya Liu

  • 1Hypertension Research Lab, Beijing Institute of Heart, Lung, Vascular Disease, Beijing Anzhen Hospital, Capital University of Medical Science, Beijing 100029, China.

Zhonghua Xin Xue Guan Bing Za Zhi
|January 22, 2008
PubMed

Insights

Reduced hyperplasia suppressor gene (HSG) expression was observed in hypertensive patients, suggesting HSG may inhibit vascular smooth muscle cell proliferation and blood pressure.

Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Hypertension Research

Background:

  • Vascular smooth muscle cell (VSMC) proliferation is implicated in hypertension.
  • The role of hyperplasia suppressor gene (HSG) in hypertension is not well understood.

Purpose of the Study:

  • To investigate HSG expression in VSMCs from normotensive and hypertensive patients.
  • To explore the correlation between HSG expression and hypertension-related parameters.

Main Methods:

  • Studied coronary artery bypass graft (CABG) patients, divided into hypertensive (n=28) and normotensive (n=26) groups.
  • Measured HSG expression in white blood cells (WBC), explanted vessels, and cultured VSMCs using Semi-Quantitative RT-PCR.
  • Assessed serum biochemical and vasoactive peptide levels.

Main Results:

  • Hypertensive patients had higher Body Mass Index (BMI) (P < 0.01).
  • HSG expression was significantly lower in WBC, VSMCs, and vessel tissue of hypertensive patients (P < 0.05).
  • HSG expression in tissue negatively correlated with BMI, systolic blood pressure (SBP), and diastolic blood pressure (DBP) (P < 0.05).

Conclusions:

  • Reduced HSG expression in hypertensive patients suggests a potential role in disease development.
  • HSG may act as an inhibitor of VSMC proliferation and blood pressure regulation.
  • Further research is warranted to elucidate HSG's precise function in hypertension.
Abstract

More Related Videos

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

Isolation of Murine Coronary Vascular Smooth Muscle Cells08:24

Isolation of Murine Coronary Vascular Smooth Muscle Cells

Published on: May 30, 2016

Related Concept Videos

Hypertension II: Pathophysiology01:29

Hypertension II: Pathophysiology

Hypertension is a chronic condition in which the blood's force against artery walls is excessively high, posing risks such as heart disease. The condition's underlying mechanisms involve complex interactions among the cardiovascular, kidney, and autonomic nervous systems.Renin-Angiotensin-Aldosterone System (RAAS): This system significantly influences blood pressure regulation. When blood pressure decreases, the kidneys secrete renin. This enzyme transforms angiotensinogen, a plasma protein,...
Antihypertensive Drugs: Vasodilators01:23

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...